Publication details
Molecular typing of exfoliative toxin-producing <I>Staphylococcus aureus</I> strains involved in epidermolytic infections
| Authors | |
|---|---|
| Year of publication | 2003 |
| Type | Article in Periodical |
| Magazine / Source | International Journal of Medical Microbiology |
| MU Faculty or unit | |
| Citation | |
| web | |
| Field | Genetics and molecular biology |
| Keywords | Bacterial Typing Techniques; DNA; Staphylococcus aureus; Exfoliative Toxin; Nucleic Acid Hybridization; Ribotyping; Pulsed Field-Gel Electrophoresis |
| Description | Pulsed-field gel electrophoresis, ribotyping, PCR ribotyping as well as prophage carriage were used for the study of genetic relationship in a set of sixteen exfoliative toxin (ET) producing strains of Staphylococcus aureus isolated from infants affected by an epidermolytic disease in two maternity hospitals in the Czech Republic. These molecular techniques made it possible to distinguish unambiguously the genetically unrelated ET-positive strains. The comparison of various genomic profiles resulted in the determination of nine different genotypes. Three strains secreted combined ETA+ETB, and the others produced ETA and enterotoxin C or TSST-1. Evidence was given that the isolates causing the epidermolytic diseases disseminated in both the maternity hospitals did not originate from a single source or the common ancestor. Close proximity of neonates to a person colonised or infected by ET-positive staphylococci seems to be the most presumptive risk factor associated with the emerged impetigo infections. |
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