Publication details
Elektrochemická detekce interakcí chinoxalinových a antracyklinových antibiotik s molekulami DNA.
| Title in English | Electrochemical detection of the interactions of chinoxaline and antracykline antibiotics with DNA |
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| Authors | |
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | Book of Abstracts. XXIV.dny lékařské biofyziky |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | DNA-echinomycin interactions; electrochemical impedance spectroscopy; mercury film electrodes; differential capacitance of the electrode double layer; |
| Description | The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins. |
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