Hydrophilic particles designed for PCR applications

• Authors: ŠPANOVÁ Alena; SOUDKOVÁ Eva; RITTICH Bohuslav; HORÁK Daniel
• Year of publication: 2003
• Type: Article in Proceedings
• Conference: 3rd International Symposium on Separations in BioSciences SBS 2003, 100 Years of Chromatography
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: magnetic microspheres; magnetite; PCR; inhibition
• Description: Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-coGMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some components used in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interferingmagnetic microspheres.

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