Publication details
Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reaction applications
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Chromatography B |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | Polymerase chain reaction inhibition; Magnetic microspheres; Methacrylates |
| Description | Magnetic hydrophilic nonporous P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA microspheres were prepared by dispersion (co)polymerization of HEMA and EDMA or GMA in the presence of several kinds of magnetite.It was found that some components used in the preparation of magnetic carriers interfered with PCR. DNA isolated from bacterial cells of Bifidobacterium longum was used in PCR evaluation of non-interfering magnetic microspheres.Preferably, electrostatically stabilized colloidal magnetite (ferrofluid) should be used in the design of new magnetic mathacrylate-based microspheres by dispersion polymerization. |
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