Magnetic hydrophilic methacrylate-based polymer microspheres designed for PCR-ready DNA isolation

• Authors: KŘÍŽOVÁ Jana; ŠPANOVÁ Alena; RITTICH Bohuslav; HORÁK Daniel
• Year of publication: 2004
• Type: Article in Proceedings
• Conference: Book of Abstarcts
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: Magnetic microspheres; Polymerase chain reaction; Methacrylates; Carboxyl groups
• Description: Newly designed magnetic nonporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), poly(glycidyl methacrylate) - PGMA and P(HEMA-co-GMA) microspheres carrying carboxyl groups were used for isolation of genomic DNA. Microspheres were prepared by radical dispersion copolymerization of respective monomers in the presence of colloidal iron oxides. DNA isolated from bacterial cells of Bifidobacterium longum by magnetic microspheres was used for PCR testing.

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