Publication details
Synthesis of mitochondrial uncoupling protein in brown adipocytes differentiated in cell culture.
| Authors | |
|---|---|
| Year of publication | 1990 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Biological Chemistry [IF=6.698 (1989); SCI #cit.<61>] |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | biogenesis; brown adipose tissue; thermogenic mitochondria; uncoupling protein |
| Description | Differentiation of precursor cells isolated from mouse brown adipose tissue was studied in cell culture, in order to characterize the biogenesis of unique thermogenic mitochondria. Synthesis of mitochondrial uncoupling protein (UCP), F1-ATPase, and cytochrome oxidase was examined by L-[35S]methionine labeling and immunoblotting. In these cells, it was possible, by using norepinephrine, to induce specifically the synthesis of the UCP but not of F1-ATPase or cytochrome oxidase. |