Publication details
Optimalizace přípravy vzorku pro dvourozměrnou elektroforézu
| Title in English | Determination fo optimal conditions for sample preparation fro two-dimensional electrophoresis |
|---|---|
| Authors | |
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Chemicke listy |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | proteomics; two-dimensional electrophoresis; sample preparation; solubilization |
| Description | The aim of this study was to set up optimal conditions for preparation of the sample of leukemic BM2 monoblasts to get a high number of resolved proteins on two-dimensional gel. Addition of thiourea in the lysis and rehydratation buffers and sonication of the sample increased the number of protein spots resolved in two-dimensional gel about 8-fold. |
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