Publication details
Identification and mapping of resistance genes to powdery mildew in barley.
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | Czech Journal of Genetics and Plant Breeding |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Barley; Hordeum vulgare; powdery mildew; gene mapping; microsatellite |
| Description | The introduction of resistance genes to powdery mildew (Blumeria graminis f. sp. hordei) present in two accessions (PI354949 and PI466495) of wild barley (Hordeum vulgare subsp. spontaneum) into H. vulgare was performed by the cross with the variety Tiffany. Powdery mildew resistance tests on plants of F2 generation revealed the segregation ratio 15 : 1 in Tiffany x PI354949 and 3 : 1 in Tiffany x PI466495, which indicated presence of two and one dominant resistance genes, respectively. To identify individual R genes, technology of microsatellite DNA markers and linkage recombination analysis was applied. One of resistance genes in PI354949 was determined to be located in Mla locus linked with Bmac0213 (25.0 cM in distal position) on chromosome 1H and the other on chromosome 7H linked with Bmag0507 (14.0 cM in proximal position). In PI466495, R gene was linked with Bmac0213 (8.4 cM in distal position) and it is also located in Mla locus. The perspective aim of this work is fine-mapping of the promising gene located on 7H chromosome and identification of tightly linked DNA markers for marker assisted selection. |