Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Fluorescently Labeled Peptides for Optimization of Laser Induced Fluorescence Detection

• Authors: VRÁBEL Patrik; TÁBORSKÝ Petr; RYVOLOVÁ Markéta; HAVEL Josef; PREISLER Jan
• Year of publication: 2004
• Type: Article in Proceedings
• Conference: Journal of Peptide Science, 3rd International and 28th European PEPTIDE Symposium
• MU Faculty or unit: Faculty of Science
• web: http://www3.interscience.wiley.com/cgi-bin/jissue/109608640
• Field: Analytic chemistry
• Keywords: electrophoresis; MALDITOF MS; fluorescence; derivatization; rhodamine B isothiocyanate; peptides
• Description: Laser induced fluorescence detection (LIF) is an effective for the sensitive determination of biologically important molecules, such as peptides and proteins, after Capillary Electrophoresis (CE) separation. In this work we demonstrate preliminary results obtained using CELIF and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) to investigate derivatization of model peptides with rhodamine-based reagents.

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