Magnetic hydrophilic methacrylate-based polymer microspheres for genomic DNA isolation

• Authors: KŘÍŽOVÁ Jana; ŠPANOVÁ Alena; RITTICH Bohuslav; HORÁK Daniel
• Year of publication: 2005
• Type: Article in Periodical
• Magazine / Source: Journal of Chromatography A
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: Magnetic and non-magnetic microspheres; carboxyl groups; DNA adsorption
• Description: Carboxyl groups containing magnetic and non-magnetic microspheres were used for isolation of genomic DNA. DNA from chicken erythrocytes and DNA isolated from bacterial cellsof Bifidobacterium longum was used for testing of adsorption/desorption properties of magnetic microspheres. The particles were used for isolation of DNA from different dairy products followed by PCR identification of Bifidobacterium strains.

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