Publication details
Analýza fenotypu a kinetiky subpopulací CD34+ buněk a lymfocytů dárců periferních hematopoetických kmenových buněk pro alogenní transplantace.
| Title in English | Analysis of phenotype and kinetics of CD34+ cell subpopulations and lymphocytes of donors of peripheral hematopoietic stem cells for allogeneic transplantation. |
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| Authors | |
| Year of publication | 2003 |
| Type | Article in Periodical |
| Magazine / Source | Časopis lékařů českých |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | CD34+ cells; immunophenotype; allogeneic transplantation |
| Description | Amount of CD34+ cells is a critical parameter for quality assessment and successful engraftment of grafts of peripheral blood hematopoietic stem cells (PBSC) in transplantation of hematopoiesis. CD34+ cells are routinely analyzed by immunophenotyping in PBSC and peripheral blood during mobilization, as well. Other leucocyte subpopulations are not usually measured. The authors present results of immunophenotyping of subpopulations of CD34+ cells and leucocytes in donors of PBSC for allogeneic transplantations, who were stimulated with the growth factor G-CSF at dose 16 mg/kg/day. The amount of CD34+ cells was not significantly different between days 4 and 5, however, there was a significant drop on day 6. CD34+90+ and CD34+61+ subpopulations reached their maximum day 4; partially differentiated CD34+ cells with co-expression of CD33, CD19, and CD7 reached maximum on day 6. CD4+ Th-lymphocytes were concentrated in the grafts during leukapheresis, CD4/CD8 ratio in the grafts was increased to average 3,06. The knowledge of kinetics of CD34+ subpopulations, together with stem cell selection and ex vivo manipulation, may have an impact on the speed of engraftment or GvHD prevention in transplanted patients. |
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