Publication details
Cytoskeleton during the cell division cycle of the yeast Cryptococcus laurentii
| Authors | |
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| Year of publication | 2005 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The cytoskeleton was investigated in Cryptococcus laurentii. The actin cytoskeleton stained by rhodamine-phalloidin in encapsulated strain CCY 17-3-5 failed, but the actin patches were detected by application of monoclonal anti-actin antibody and secondary antibody SwAM-FITC. In acapsular strain C. laurentii 17-3-6, F-actin was stainable by rhodamine-phalloidin. The cortical actin patches were distributed under cell surface and actin cables extended to the bud. At cytokinesis, the actin cables were replaced with contractile ring. Microtubules were seen in both yeast strains. Cytoplasmatic microtubules during interphase arranged into a random network under the plasma membrane. Before mitosis, nucleus migrated from the mother cell to the bud, cytoplasmatic microtubules were disassembled, the spindle formed that allowed the nuclear division. Before the cytokinesis the cytoplasmatic microtubules reappeared. The dynamics of cytoskeleton in C. laurentii is similar to Cryptococcus neoformans (2). |
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