Publication details
Hybridní molekuly kolicinů U/Y a jejich interakce s imunitními proteiny
| Title in English | Hybrid colicin U/Y Molecules and their interactyion with immunity proteins |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | Sborník abstraktů XIII. konference mladých mikrobiologů Tomáškovy dny 2004 |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | colicin; immuinity proteins |
| Description | Sixteen hybrid U/Y proteins were constructed. Three amino acid residues of colicin U and four amino acid residues of colicin Y were found to be important for interaction with cognate immunity proteins. The amino acid residues F576, G580 and L594 were found to be involved in the interaction between colicin U and its immunity protein. Another interaction region is located in the position 590-592. The amino acid residues Y586, V590, A594 and A595 were identified in recognition of colicin Y by its cognate immunity protein. In addition, one of the 34 amino acids in colicin Y C-terminal region (12 amino acids are different when compared to sequence of colicin U) had a slight effect in the recognition of colicin Y with its immunity protein. Important amino acid residues of colicin U and Y interacting with cognate immunity proteins were found to be located in the hydrophobic sequence between helices 8 and 9 in the colicin pore-forming domain. |
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