Publication details
Aeromonas hydrophila ssp. dhakensis - first occurrence in Czech Republic confirmed by ribotyping
| Authors | |
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| Year of publication | 2005 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Aeromonads are a new unusual pathogens, which can cause mainly gastroenteritis as well as wound infection or septicaemia. Identification of aeromonads to the species level is due to ever changing taxonomy very difficult and sometimes doubtful. Recently, three subspecies of A. hydrophila - A. hydrophila ssp. hydrophila, A. hydrophila ssp. dhakensis and A. hydrophila ssp. ranae have been described. Aim of the study was to prove if the mentioned taxons can be isolated from clinical samples and identified in routine clinical laboratory and if also A. hydrophila ssp. dhakensis could be involved in diarrheal diseases in our region. 138 isolates of aeromonads were obtained in routine survey in 2003 and 2004. Biochemical identification was done by ENTEROtest24 (Pliva-Lachema), nevertheless the supplementary conventional biochemical tests (acid production from arabinose, salicin, arbutin, hydrolysis of aesculin) were done. Results were evaluated by software TNW (Pliva-Lachema). Cellular fatty acid analysis was performed by MIS Sherlock (MIDI, Inc., USA). Ribotyping of selected isolates and reference cultures using EcoRI restriction enzyme and DNA probe complementary to 16S and 23S rRNA was accomplished. From 138 aeromonads 35 A. hydrophila isolates were found. Biochemical identification of isolates based on commercial kit was often uncertain due to variations observed in some tests and required additional testing. Among them, only one strain from patient who probably acquired the infection in Egypt, was identified as A. hydrophila ssp. dhakensis on the base of ribotyping. All other isolates were identified as A. hydrophila ssp. hydrophila, none of A. hydrophila ssp. ranae was proved. Species identification was confirmed also by fatty acid analysis. The less frequent A. hydrophila ssp. dhakensis was occurred in our region. Use of an appropriate identification approach can easily warn microbiologist of a rare species. Diarrhoea due to A. hydrophila ssp. dhakensis was an imported case of infection and ribotyping appeared to be a good tool for the identification of aeromonads. |
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