Publication details

Application of PCR, rep-PCR and RAPD techniques for typing of Lactococcus lactis strains

Authors

PRODĚLALOVÁ Jana ŠPANOVÁ Alena RITTICH Bohuslav

Year of publication 2005
Type Article in Periodical
Magazine / Source Folia Microbiologica
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Lactococcus lactis; PCR; rep-PCR; RAPD
Description A collection of 34 lactococcal strains were characterized using PCR for acmA gene, and for 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindromes-PCR (rep-PCR). PCR experiments enabled the genotypic identification of L. lactis strains by RAPD. Rep-PCR did not distinguish between L. lactis subspecies. In some cases, phenotypic classification of L. lactis subspecies did not correlate with genotypic characterizations.
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