Publication details
Functional polymer hydrogels for embryonic stem cell support
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Biomedical Materials Research |
| MU Faculty or unit | |
| Citation | |
| Field | Macromolecular chemistry |
| Keywords | hydrogel; cationic; embryonic stem cells; 2-hydroxyethyl methacrylate; 1-vinyl-2-pyrrolidone |
| Description | One of the most exciting challenges in development of transplantation therapies is to repair the damaged part of the organ or tissue by transplantation of undifferentiated embryonic stem (ES) cells or their differentiated derivatives within three-dimensional polymer scaffold. To address this issue, new cationic hydrogel slabs were synthesized by bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) with ethylene dimethacrylate (EDMA) or 1-vinyl-2-pyrrolidone (VP) with N,N'-divinylethyleneurea (DVEU) or EDMA in the presence of saccharose or NaCl as a porogen. Biocompatibility was studied with the use of feeder-independent mouse ES cell line D3. When cultured for a limited period of time, ES cells retained their undifferentiated state independently of properties of the hydrogel slabs. Notably, prolonged culture in superporous hydrogel slabs initiated ES cell differentiation. |