Publication details

Neural differentiation of pluripotent mouse embryonal carcinoma cells by retinoic acid - inhibitory effect of serum

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Authors

PACHERNÍK J. BRYJA V. EŠNER M. KUBALA L. DVOŘÁK Petr HAMPL Aleš

Year of publication 2005
Type Article in Periodical
Magazine / Source Physiol. Res.
MU Faculty or unit

Faculty of Medicine

Citation
Field Genetics and molecular biology
Keywords cells
Description This study employs mouse embryonal carcinoma (EC) cells P19 to investigate the effects of serum on RA-induced neural differentiation occurring in a simplified monolayer culture. The data show that mouse EC cells are able to produce neural cells upon exposure to RA even without culture in three-dimensional embryoid bodies (EBs). However, in contrast to standard EBs-involving protocol(s), neural differentiation in monolayer only takes place when complex signaling from serum factors is avoided. This simple and efficient strategy is proposed to serve as a basis for neurodifferentiation studies in vitro.
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