Publication details
Detection of chromosome 13 abnormalities and 14q32 translocations in multiple myeloma using simultaneous imunofluorescent labelling of malignant plasma cells and fluorescent in situ hybridization
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | European Journal of Human Genetics |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Multiple myeloma; chromosome aberrations; FISH; FICTION |
| Description | Chromosomal aberrations such as 13q14 deletions or translocations involving 14q32 are described to be common cytogenetic findings in multiple myeloma (MM). Especially, deletions of 13q14 has been associated with an adverse outcome and it has been proposed as one of the most important prognostic factors for MM patients. Because metaphase cytogenetic studies in MM are hampered by a low proliferative activity of myeloma cells in vitro, interphase fluorescent in situ hybridization (FISH) using specific DNA probes is the technique most widely used for the determination of genomic aberrations in this disease. In the present study we have performed fluorescence in situ hybridization experiments with probes directed to the 13q14 and 14q32 chromosomal regions in 30 patients with MM. For identification of malignant plasma cells in bone marrow samples, we have used cytoplasmic immunoglobulin (cIg) labelling methodology (Ahmann et al. 1998) This method allowed us to identify simultaneously monotypic plasma cells by monoclonal antibody fluorescence (anti-Ű or anti-Ü) and detect chromosomal abnormalities by FISH (cIg-FISH). FISH studies revealed that monoallelic deletions of 13q14 or monosomy 13 were present in 15 of 30 (50 %) patients, 14q32 abnormalities were observed in 9 of 30 (33 %) patients with MM. Our results confirmed that by combining immunofluorescent labelling of myeloma cells and FISH, 13q14 deletion can be proved also in patients with apparently normal karyotype in unselected bone marrow sample. We conclude that cIg-FISH procedure represents simple and reliable methods that can increase the incidence of chromosomal abnormalities required for prognostic evaluations of patients with MM. |