Publication details

Genotypic characterisation of vancomycin-resistant <I>Enterococcus faecium</I> isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic

Authors

KOLÁŘ Milan PANTŮČEK Roman VÁGNEROVÁ Iva KESSELOVÁ Michaela SAUER Pavel MATOUŠKOVÁ Ivana DOŠKAŘ Jiří KOUKALOVÁ Dagmar HEJNAR Petr

Year of publication 2006
Type Article in Periodical
Magazine / Source Clinical Microbiology and Infection
MU Faculty or unit

Faculty of Science

Citation
Web [Abstract, Full text]
Field Genetics and molecular biology
Keywords Enterococcus faecium; molecular epidemiology; pulsed-field gel electrophoresis; vancomycin-resistant enterococci
Description This study describes the first molecular characterisation of clinical isolates of vancomycin-resistant enterococci (VRE) in the Czech Republic. Of 2647 patient isolates of Enterococcus spp. from 1997-2002, 121 (4.6%) were identified as VRE. The most common isolates were VanA+ Enterococcus faecium (78%) and VanB+ Enterococcus faecalis (10%). In addition, five VanA+ E. faecium isolates were obtained from environmental and staff sampling. Macrorestriction analysis of SmaI restriction fragment length polymorphism was performed for 54 VanA+ E. faecium clinical isolates and the five VanA+ E. faecium environmental isolates. Thirty-two unique restriction endonuclease patterns were identified, including two predominant clonal types represented by five or more isolates. Two environmental VanA+ E. faecium isolates were closely related to two patient isolates, which had an identical SmaI macrorestriction pattern. The results indicated potential survival of strains in the hospital environment and possible subsequent transmission to hospitalised patients.
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