Publication details
Chromatographic analysis of naphthoquinones in plants
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Chemické Listy |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | HPLC; In-vitro cultires; Dionaea-muscipula; Plumbagin; Rotundifolia; Juglone; Lawsone |
| Description | The aim of the work was to optimize the high performance liquid chromatography coupled with a diode array detector for the determination of naphthoquinones (1,4-naphthoquinone, lawsone, juglone and plumbagin). The optimal conditions were: mobile phase 0.1 mol l(-1) acetic acid - methanol (35: 65 v/v), flow rate 0.8 ml min(1), column and detector temperature 40 C. Detection limits (3 S/N) were 65 ng ml(-1), 50 ng ml(-1), 75 ng ml-1 and 39 ng ml-1 for lawsone, 1,4-naph-tho-qui-none, juglone and plumbagin, respectively. The optimized method was used for the determination of the mentioned naphthoquinones in plants (Dionaea muscipula, Drosera rotundifolia, Drosera spathulata, Drosera capensis and Paulownia tomentosa). |