Publication details
Počítačové hodnocení intenzity sekrece antigenů systému AB0 do slin
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Tomáškovy dny 2006 |
| MU Faculty or unit | |
| Citation | |
| Description | Introduction: We can divide human population into secretors and non-secretors based on presence or absence of ABO blood group specific substances in body fluids. The ability to secrete is independent of the AB0 genes which determine blood type but is determined by secretor genes Se/se which are inherited as a Mendelian character. Secretors have SeSe or Sese genotypes; the genotype sese belongs to non-secretors. The aim of research was blood typing of a sample set human saliva, programming an application and using this application for assessment of agglutination intensity and description of the secretor phenomenon. Materials and Methods: The set of 138 saliva samples was tested by serological method. Saliva was acquired from people of both sexes and of a similar age group. We used the modified absorption inhibition method for the blood typing. Each sample was tested in 12 tubes four tubes for each antigen A, B and H. Presence of antigen is shown as inhibition of agglutination. The amount of antigens in saliva is indirectly proportional to the intensity of agglutination. The results of agglutination were scanned and we counted the area of agglutinated clusters of each sample by using the application for agglutination intensity. This application uses a graphic model HLS (Hue, Lightness, Saturation) for recognition of agglutinated clusters from red blood cell suspension. Results: Presents of the antigens and their amount are independent of sex. The intensity of secretion of the antigens ABH is different in various blood groups. The intensity of secretion into the same body fluid is individually dependent even within the same blood group. This variability is determinated by Se/se genes. Allele Se is incompletely dominant over allele se. It causes bimodal distribution of secretion intensity and secretors can be divided into two groups stronger secretors (with genotype SeSe) and weaker secretors (with genotype Sese). Non-secretors have genotype sese. Conclusions: Main contribution of the research is creation of a software for evaluation of agglutination intensity which can replace visual evaluation. The evaluation performed by this computer application is independent of the outside conditions. The application eliminates a subjective error in the visual evaluation, because it recognizes smaller clusters than a human eye does and therefore the evaluation performed by the application is more exact. It allows using fewer tubes and continuous results allow more effective usage of statistical methods. |