Použití magnetických mikročástic pro transkriptomovou analýzu

• Title in English: Using of magnetic micro-beads for analysis of transcriptome
• Authors: HUŠKA Dalibor; PETRLOVÁ Jitka; ADAM Vojtěch; TRNKOVÁ Libuše; HAVEL Ladislav; KIZEK René
• Year of publication: 2006
• Type: Article in Proceedings
• Conference: Vyziva zvirat 2006 - PROTEINY
• MU Faculty or unit: Faculty of Science
• Field: Electrochemistry
• Keywords: DNA; RNA; hybridization; electrochemical detection; adenine
• Description: A simple, rapid and low cost detection of DNA and RNA sequence is a difficult task for many scientists around the world. Here, we suggest utilizing paramagnetic micro-beads in connection with electrochemical detection of adenine to study of transcriptome (total mRNA). Moreover time of hybridization of nucleic acid on the surface of the beads has been investigated. Recovery of the analysis is sufficient.

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