Publication details
Borik asitin Galleria mellonella (Lepidoptera: Pyralidae) nýn hemolenf protein profili, lipid peroksidasyonu düzeyi ve antioksidan savunma sistemine etkisi
| Title in English | Hemolymph protein profiles, lipid peroxidation level and antioxidant defense system of G. mellonella after boric acid treatment |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | 18. Ulusal Biyoloji Kongresi |
| MU Faculty or unit | |
| Citation | |
| Keywords | G. mellonella, boric acid, protein profiles, antioxidant enzymes. malondialdehyde |
| Description | Hemolymph protein profiles, lipid peroxidation level and antioxidant defense system of the greater waxmoth, Galleria mellonella (L.) final instars and pupae were investigated by rearing the newly hatched larvae on an artificial diet containing 156, 620, 1250, and 2500 ppm of boric acid. Sodium dodecyl sulphate polyacrylamide gradient gel electrophoresis (SDS-PAGGE) was used to determine the hemolymph protein profiles. Using this technique it was possible to distinguish approximately 15 protein fractions in the final instar larva, and in pupae about 13 protein fractions were observed. In addition to well-defined protein fractions such as lipophorins (245 kDa) and storage proteins (80 kDa), some undetermined protein fractions varying from 18260 kDa were also detected. Although the changes in protein profiles of larvae in lower concentrations of boric acid were initially not detected, later, a clear influence of the highest concentration of boric acid was observed. One protein fraction of molecular weight 43 kDa was significantly bigger after the treatment. No such difference was observed in pupal hemolymph. Lipid peroxidation product, malondialdehyde (MDA) level was significantly increased after high concentrations of boric acid treatment in hemolymph of final instars. Highest concentration of boric acid (2500 ppm) markedly decreased catalase (CAT) activity from 0.32 ą 0.003 in the control to 0.04 ą 0.001 mmol/mg protein/min and glutathione S-transferase (GST) activity from 3.30 ą 0.32 to 2.59 ą 0.34 nmol/mg protein/min in larval hemolymph. Boric acid at 1250 ppm significantly decreased MDA level from 0.30 ą 0.04 in control to 0.12 ą 0.02 nmol/mg protein while GST activity was not significantly affected in pupal hemolymph. However all concentrations of boric acid significantly increased CAT activity in hemolymph of pupae This study suggests that boric acid-induced oxidative stress may be causative factor in deterioration of larval antioxidative defense system and that decreased CAT and GST activities for highest concentration of boric acid may result in appearance of a new stress protein in hemolymph of final instars. |