Publication details

Attomole voltammetric determination of metallothionein

Authors

PETRLOVÁ Jitka POTĚŠIL David MIKELOVÁ Radka BLAŠTÍK Ondřej ADAM Vojtěch TRNKOVÁ Libuše JELEN František PRŮŠA Richard KUKAČKA Jiří KIZEK René

Year of publication 2006
Type Article in Periodical
Magazine / Source Electrochimica Acta
MU Faculty or unit

Faculty of Science

Citation
Field Electrochemistry
Keywords Metallothionein; Mercury electrode; Differential pulse voltammetry; Cyclic voltammetry; Brdicka reaction; Adsorptive transfer stripping technique;Reducing agent; Tris(2-carboxyethyl)phosphine; Lead poisoning; Human blood serum
Description Number of authors have concerned with electrochemical analysis of metallothionein. Recently new electroanalytical techniques enabling determination of MT at picomole level has been suggested. The aim of the presented work was to show advantages and disadvantages of the different electrochemical procedures, which are commonly used for the detection of MT(i) cyclic voltammetry, (ii) differential pulse voltammetry, and (iii) Brdicka reaction. Primarily we aimed on improvement of the mentioned techniques. Using of reducing agent (tris(2-carboxyethyl)phosphine) and combination of the mentioned method with adsorptive transfer stripping technique (AdTS) were the main improvements of the voltammetric method. The detection limits of metallothionein measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and DPV Brdicka reaction were 0.5 pmol, 4 fmol and 10 amol, respectively. In addition AdTS DPV Brdicka reactionwas used for the determination of metallothionein in human blood serum of 11-year-old girl, which were lead poisoned.
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