Publication details
Preparation and use of monoclonal antibodies to detect Phytophthora nicotianae var. nicotianae.
| Authors | |
|---|---|
| Year of publication | 1999 |
| Type | Article in Periodical |
| Magazine / Source | Ochrana rostlin |
| MU Faculty or unit | |
| Citation | PEKÁROVÁ-KYNĚROVÁ, B. and M. KUTÍKOVÁ. Preparation and use of monoclonal antibodies to detect Phytophthora nicotianae var. nicotianae. Ochrana rostlin. Praha: Ústav zemědělských a potravinářských inf, 1999, vol. 35, No 2, p. 41-46. ISSN 1212-2580. |
| Keywords | Phytophthora nicotianae var. nicotianae; monoclonal antibodies; indirect ELISA; immunoprinting; tomato |
| Description | A monoclonal antibody (MAb 18) was prepared against purified mycelial proteins from Phytophthora nicotianae var. nicotianae. The specificity of MAb 18 (IgG class) was tested using indirect ELISA (PTA-ELISA). It cross-reacted with Phytophthora cactorum, P. cinnamomi, P. cryptogea, P. fragariae, but not with other fungi (Fusarium oxysporum, Pythium ultimum and P. oligandrum) and bacteria (Clavibacter michiganensis subsp. michiganensis) isolated from tomato. Phytophthora nicotianae var. nicotianae was detected in roots and basal stems of artificially infected young tomato plants using indirect ELISA and immunoprinting. |