Publication details
Utilization of immunochemical methods for the detection of Fusarium culmorum in winter wheat
| Authors | |
|---|---|
| Year of publication | 2000 |
| Type | Article in Periodical |
| Magazine / Source | Ochrana rostlin |
| MU Faculty or unit | |
| Citation | |
| Keywords | Fusarium culmorum; winter wheat; polyclonal and monoclonal antibodies; PTA-ELISA; imunoprinting; dot blot; imunofluorescent microscopy |
| Description | Two monoclonal antibodies and one polyclonal antibody were prepared and use for the detection of Fusarium culmorum in winter wheat. In addition, Fusarium oxysporum, F. graminearum, F. solani, F. avenaceum, F. tricinctum, and F. moniliforme were used in tests. The antibodies were genus specific. Monoclonal antibodies did not cross-react with other fungal pathogens of winter wheat (Gaeumannomyces graminis, Psudocercosporella herpotrichoides, Rhizoctonia solani). The pathogen was detected in vitro, in hydrated and artificially inoculated kernels and in seedlings, by PTA-ELISA, immunoprinting, dot blot and immunofluorescent microscopy. Dot blot and immunofluorescence microscopy were the most sensitive method for recognising FCu in vitro and in vivo. Specificity and sensitivity of monoclonal and polyclonal antibodies were similar. |