Publication details
The diagnosis of Fusarium culmorum by polyclonal antibodies - preparation and character of antigens ans antibodies.
| Authors | |
|---|---|
| Year of publication | 1997 |
| Type | Article in Periodical |
| Magazine / Source | Ochrana rostlin |
| MU Faculty or unit | |
| Citation | |
| Keywords | Fusarium culmorum, media, isolates, morphological and physiological character, antigen, proteins, polyclonal antibodies, sensitivity, specificity, cross-reactions |
| Description | Eighteen monospore Fusarium culmorum (FCu) isolates with different pathogenicity were cultivated on five liquid media. Czapek Dox and Minimal medium were suitable media. The morphological and physiological characteristics were determined (chlamydospore and conidia production, conidia dimension, character of mycelia, colour of media, growth dynamics of mycelium and weight of dry mycelial mats). The composition of media influenced morphological and physiological characters of isolates. Pathogenicity was not correlated with character of isolates. Antigens were prepared by extraction of proteins from mycelial mats in a special buffer, then purified by centrifugation and ultracentrifugation of supernatant. The composition of media influenced quantity and quality of proteins of antigens. The pathogenicity of FCu isolates was not correlated with protein content of antigens. Specificity and sensitivity of polyclonal antibodies after immunization of rabbits with FCu antigens were studied. Different reactions of antigens of FCu isolates were not found. Anti-FCu IgG reacted with antigens of another species of Fusarium (oxysporum, solani, equisetum, nivale, sambucinum, poae, avenaceum), the differences were quantitative. Cross-reactions of anti-FCu IgG with antigens of Pseudocercosporella herpotrichoides, Gaeumannomyces graminis, Rhizoctonia solani, Trichoderma viridae (pathogenic isolates of wheat) were not observed. Reaction of antisera and IgG with antigens were evaluated by agar double diffusion and ELISA. |