Publication details
Molecular markers used in phylogenetic studies of Diptera with a methodological overview
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Acta Universitatis Carolinae |
| MU Faculty or unit | |
| Citation | |
| Field | Zoology |
| Keywords | Diptera molecular markers phylogeny |
| Description | The use of DNA analysis permits to resolve many taxonomic and phylogenetic problems at different levels. In this study, we try to give a summary of used genetic markers on different taxonomic levels in the phylogenetic study of Diptera and a methodical overview of the laboratory methods. The gene choice is of crucial importance. Some of the gene markers are applicable in a wide range of taxonomic levels in the Diptera flies (16S, COI, 18S, tRNA-leucine), other are limited to lower (subgeneric or intraspecific) level (cytB). Within the gene choice, the proper DNA extraction is also very important task, what works upon a success of the whole process. |
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