Publication details
Ecotoxicity and bioavailability of phenanthrene (PHE) in polluted soils: comparison on Eisenia andrei Bouché (Oligochaeta Lumbricidae) and Galleria mellonella (Linnaeus) (Insecta Pyralidae)
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| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | The Seventh European Meeting on Environmental Chemistry EMEC7, The Book of Abstracts |
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| Citation | |
| Description | The bioavailability evaluation of pollutants in soil has recently increased the interest of the international scientific community. Total chemical analysis of contaminants in samples collected from polluted soils are usually carried out. However, the environmental risk assessed by means of these procedures may be overestimated. The aim of present contribution is to compare the acute and the chronic ecotoxicity and the bioavailability of phenanthrene (PHE) in two different test species: an earthworm, Eisenia andrei, and an insect, larvae of Galleria mellonella. A specific organic substrate and an artificial soil (OPPTS-1996 protocol) were both contaminated with 15, 30, 60, 105,150 ppm of PHE and aged for 20 days before animal exposure. The test species were placed in contact with contaminated substrate or soil in order to compare their responses to different doses of PHE. For both species LC50 and the PHE (bio)accumulation in the animal body were assessed at the end of two exposure periods (7 and 14 days). Furthermore, the influence of the pollutant on the immunological system of these two invertebrates was studied using Trypan blue dye exclusion, NRRT (Neutral Red Retention Time) lysozyme and acrylamide electrophoresis assays. The exposing procedures to contaminated artifical soils and the biological assays appear to be more suitable for the earthworm than the moth larvae. |