Publication details
Technique of light chain-specific immunofluorescent staining of clonal plasma cells and FISH analysis
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Detection of chromosomal aberrations in multiple myeloma |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Multiple myeloma; FISH; chromosomal aberrations |
| Description | MULTIPLE MYELOMA (MM) is characterized by monoclonal proliferation of plasma cells and represents approximately 10% of all hematologic malignancies. The clinical course of patients with MM is highly variable. Reliable genetic indicators are therefore required for their stratification and for more adequate therapy. The metaphase cytogenetic studies in MM are hampered by a low proliferative activity of myeloma cells in vitro. Therefore, interphase fluorescent in situ hybridization (I-FISH) using specific DNA probes is the technique of choice for the determination of genomic aberrations associated with this disease. However, I-FISH on bone marrow specimens do not distinguish malignant plasma cells. In order to solve this problem, various novel techniques have been designated for selection of myeloma cells. Immunofluorescent labelling of malignant plasma cells is suitable approach for the diagnosis of chromosomal abnormalities in patients with MM. This method allows selection of monotypic plasma cells by monoclonal antibody fluorescence (anti-kappa or anti- lambda) and detection of chromosomal abnormalities by interphase FISH (cIg FISH). Another possible way of detection plasma cells is their purification on magnetic activated cell sorting (MACS). In our presentation we show single steps of immunofluorescent labelling procedure and we compare the results of cytogenetic analyses performed by cIg-FISH method with FISH analyses performed on MACS. According to our experience, based on our research and present conditions of our laboratory, the cIg FISH represents cheaper and more effective method than MACS. |
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