Publication details
Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Analytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | cathodic stripping voltammetry; graphite electrodes; DNA hybridization; magnetic nanoparticles; oligodeoxynucleotides; |
| Description | A simple, cost-effective design for amplifying oligodeoxynucleotide (ODN) sensing, in microliter ODN volumes containing copper ions, by solution streaming ( bubbling)is presented. The inert gas streaming ( bubbling) at a constant pressure of 0.04 bar drives the motion of a 30 microliter ODN droplet containing a three-electrode circuit ( inverted drop microcell), and in the presence of copper ions offers an similar to 50-times improvement in the detection of ODN samples. The detection of ODNs at the carbon paste electrode is based on the enhancement of the oxidation peaks of purine bases ( adenine and guanine) by the anodic stripping of the electrochemically accumulated complex of Cu(I) with purine base residues of acid hydrolyzed ODN samples ( Cu(I)-ahODN complex). |
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