Publication details

Isolation and characterization of microsatellites in Neogobius kessleri (Perciformes, Gobiidae) and cross-species amplification within the family Gobiidae

Authors

VYSKOČILOVÁ Martina ONDRAČKOVÁ Markéta ŠIMKOVÁ Andrea MARTIN Jean-François

Year of publication 2007
Type Article in Periodical
Magazine / Source Molecular Ecology Notes
MU Faculty or unit

Faculty of Science

Citation
Field Zoology
Keywords Neogobius; Proterorhinus; Gobiidae; microsatellites; primers; cross-amplification
Description Fourteen polymorphic microsatellites were isolated from Neogobius kessleri, a benthic fish of Ponto-Caspian origin which has been recently introduced into the Middle and Upper Danube River. Number of alleles and heterozygosity per locus in a sample of 32 fish individuals ranged from two to four and from 0.13 to 0.75, respectively. These primers will be useful in determining the population structure of N. kessleri. In addition, successful cross-amplification was obtained for four related species, N. melanostomus, N. fluviatilis, N. gymnotrachelus and Proterorhinus marmoratus. These microsatellite loci may be useful for the evaluation of the origin of non-native goby populations.
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