Publication details
Utilizing of electrochemical detection coupled with liquid chromatography for isoflavones detection
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Acta Chim. Slov. |
| MU Faculty or unit | |
| Citation | |
| Field | Plant production |
| Keywords | electrochemical detection; liquid chromatography; isoflavones |
| Description | Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min-1; column and detector temperature: 26 C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL-1. Their simultaneous determination takes 15 min. |
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