Publication details
Study of structural changes of lactoferrin using flow injection analysis with electrochemical detection on the surface of glassy carbon electrode
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Acta Chim. Slov. |
| MU Faculty or unit | |
| Citation | |
| Field | Plant production |
| Keywords | structural changes; lactoferrin; flow injection analysis; electrochemical detection; glassy carbon electrode |
| Description | Lactoferrin is considered to be a multifunctional protein. It appears to play several biologically important roles, where its structure is very crucial. The aim of this work was to investigate the basic electrochemical behaviour of lactoferrin by both stationary and flow electrochemical methods with respect its structural changes under various denaturing conditions. The electroactivity of lactoferrin was studied by three various electrochemical methods (linear sweep, differential pulse and square wave voltammetry). Based on the results obtained, we utilized flow injection analysis with electrochemical detection (FIA ED) for determination of lactoferrin. We found out that the most suitable FIA ED conditions were as follows: working electrode potential of 900 mV, Britton Robinson buffer (pH 4.5) as the mobile phase, its flow rate 1 ml min 1. Finally, we attempted to follow the changes of lactoferrin signal in the presence of chemical compounds or under the physical conditions leading to changes in its structure. As we have shown here, electrochemical analysis enables us to distinguish changes of protein structure easily and rapidly. |
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