Publication details
Inhibition of endocytosis blocks Wnt signalling to beta-catenin by promoting dishevelled degradation
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Acta Physiologica |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | beta-catenin; clathrin-mediated endocytosis; desensitization |
| Description | We investigated the effects of endocytosis inhibition on Frizzled4-green fluorescent protein (FZD4-GFP) localization, dishevelled levels and Wnt-3a signalling to beta -catenin. Hyperosmotic sucrose and K+ depletion increased the membrane localization of FZD4-GFP, and in parallel triggered fast (1–2 h) and almost complete (approx. 95%) degradation of endogenous dishevelled, which further prevented canonical signalling by Wnt-3a to beta-catenin. The data provide evidence for a molecular mechanism that could be a basis for a novel negative feedback loop within the Wnt/Frizzled pathway depending on dishevelled degradation. |