An increased invasion of ED-1+ cells into ipsi- and contralateral DRG following sciatic nerve ligature and ventral root transection

• Authors: TUČKOVÁ Lucie; DUBOVÝ Petr; JANČÁLEK Radim; SVÍŽENSKÁ Ivana; KLUSÁKOVÁ Ilona
• Year of publication: 2007
• Type: Appeared in Conference without Proceedings
• MU Faculty or unit: Faculty of Medicine
• Description: Macrophages increase in number in dorsal root ganglion (DRG) following nerve injury, and their activities are referred as to induce neuropathic pain. Macrophages exhibiting ED-1+ immunostaining were examined quantitatively in lumbar ipsi- (iDRG) and contralateral (cDRG) ganglia of naive rats and those following nerve injury. An amount of ED-1+ macrophages were found in naive DRG without intimate location to the neuronal bodies. ED-1+ was significantly enlarged and located close to neuronal bodies in iDRG 2 and 4 weeks from sciatic nerve ligature. cDRG also displayed an increase amount of ED-1+ macrophages, predominantly following 4 weeks. Ventral root transection resulted in an increased amount of ED-1+ cells in both i- and cDRG. However, the enlargement was similar on both sides after 2 weeks, but greater elevation was observed only in iDRG after 4 weeks. The results indicate that nerve injury stimulated invasion of ED-1+ macrophages predominantly into iDRG, and later to cDRG, and suggested that Wallerian degeneration produces signals for invasion of macrophages to cDRG.

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