Publication details
Kvantifikace redukovaného a oxidovaného glutathionu ve spermiích určitých druhů ryb
| Title in English | Quantification of reduced and oxidized glutathione in sperm of certain fish species |
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| Authors | |
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | The First International Workshop on the Biology of Fish Sperm |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Heavy metals; fish sperm; thiol compounds |
| Description | It is known that heavy metals, first of all iron (Fenton reaction), generate the reactive oxygen species in the organism. The oxidation stress caused the severe damage of DNA and biological membranes. Sperm have a high content of polyunsaturated fatty acids in membranes and high amount of DNA, which are sensitive to oxidative stress. The oxidation damaging of the membranes and DNA in the sperm can influence the process of fertilization and embryogenesis. The aim of our work was to develop a simple and quick method for determination of GSH and GSSG content in sperms of certain fish species using high performance liquid chromatography with electrochemical detection (HPLC-ED). |