Publication details
Comparison of spectrophotometry to electrochemical methods of urease determination
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | Chemické zvesti |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | Electrochemistry; Spectrophotometry; Urease |
| Description | Enzyme urease is ubiquitous enzyme in plants and is also present in many eucaryote microorganisms and bacterias. It consists of 840 aminoacids residues and molecular weight is higher than 90 kDa. It playse a key role in catalysis of dissociation of urea while the formation of carbon dioxide and ammonia. For enzyme activity analysis was developed a series of direct and indirect analytical methods. The most used are the detection of ammonia by reaction with indophenol, determination 14CO2 and series of enzymatic reactions coupled with spectrophotometric measurement of NADPH oxidation. The aim of this study was to compare spectrophotometric detection of urease activity completed by Berthelot reaction and electrochemical detection of reduction signals of urease and Brdička catalytic signals. |