Publication details
Coupling of monolithic column sample preparation and flow injection analysis - Electrochemical detection for determination of lactoferrin: easy, low cost and structural distuinguishing
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007 |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Lactoferrin; flow ingection analysis; electrochemical detection |
| Description | Lactoferrin is a glyco- and iron-binding protein consisted from 703 aminoacid residues. Its molecular weight is of about 77 000 Da. Except iron lactoferrin is able to bind other heavy metals into the same binding place, but with lower affinity. The main biological function of lactoferrin is to maintain iron homeostasis. Iron capturing occurs primary in the intestine from food. It is related to cell growth regulation, cell differentiation and it has an antiinflammatory activity. The main aim of this work was to suggest a method to distinguish structure of lactoferrin easily and with low demanding on instruments and costs. As detection part of the method we used flow injection analysis with electrochemical detection. |