Brain metabolism and diffusion in the rat cerebral cortex during pilocarpine-induced status epilepticus

• Authors: ŠLAIS Karel; VOŘÍŠEK Ivan; ZOREMBA Norbert; HOMOLA Aleš; DMYTRENKO Lesia; SYKOVÁ Eva
• Year of publication: 2008
• Type: Article in Periodical
• Magazine / Source: Experimental Neurology
• MU Faculty or unit: Faculty of Medicine
• Field: Pharmacology and pharmaceutical chemistry
• Keywords: Diffusion; ADCW; Microdialysis; Pilocarpine; Epilepsy; Glucose; Glutamate; Pyruvate; Lactate; Potassium
• Description: The real-time iontophoretic method using tetramethylammonium-selective microelectrodes and diffusion-weighted magnetic resonance imaging were used to measure the extracellular space volume fraction, tortuosity and apparent diffusion coefficient of water (ADCW) 240 min after the administration of pilocarpine in urethane-anaesthetized rats. The obtained data were correlated with extracellular lactate, glucose, and glutamate concentrations and the lactate/pyruvate-ratio, determined by intracerebral microdialysis.

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