Publication details

Real-time PCR diagnostics failure caused by nucleotide variability withing exos 4 of human cytomegalovirus major immediate-early gene

Authors

LENGEROVÁ Martina RÁČIL Zdeněk VOLFOVÁ Pavlína LOCHMANOVÁ Jana BERKOVCOVÁ Jitka DVOŘÁKOVÁ Dana VORLÍČEK Jiří MAYER Jiří

Year of publication 2007
Type Article in Periodical
Magazine / Source Journal of Clinical Microbiology
MU Faculty or unit

Faculty of Medicine

Citation
Field Oncology and hematology
Description Here we report how variability in the human cytomegalovirus genome sequence may seriously affect the outcome of its molecular diagnosis. A real-time quantitative PCR assay targeting the major immediate-early gene failed to detect the viral load in some, but not all, clinical samples from hematooncological patients. By amplification and sequencing the DNA across the regions targeted by this assay we found a number of nucleotide substitutions which accounted for decreased primer/probe binding. This decreased the sensitivity of the assay up to 1,000-fold.

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