Publication details
Detekce invazivní aspergilózy pomocí PCR a real-time PCR - přednosti a úskalí
| Title in English | Detection of invasive aspergillosis by PCR and real-time PCR--benefits and drawbacks |
|---|---|
| Authors | |
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Klinická mikrobiologie a infekční lékařství |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | PCR; real-time PCR; Aspergillus sp.; invasive aspergillosis |
| Description | Although PCR detection of mycotic pathogens in clinical samples has been around for almost two decades using of this method for diagnosis of invasive aspergillosis is still controversial. Molecular methods are routinely used in various fields of modern microbiology, e. g. genotyping of bacterial strains resistant to antibiotics, molecular epidemiology or routine detection of some viruses. PCR methods made the diagnostics faster, accurate and more feasible. This review intends to show why molecular diagnostics of invasive mycotic infections is so tricky and highlight the main limiting factors. |