Publication details

Complete sequence of low-copy-number plasmid MccC7-H22 of probiotic Escherichia coli H22 and the prevalence of mcc genes among human E. coli.

Authors

ŠMAJS David STROUHAL Michal MATĚJKOVÁ Petra ČEJKOVÁ Darina CURSINO Luciana CHARTONE-SOUZA Edmar ŠMARDA Jan NASCIMENTO Andrea M.A.

Year of publication 2008
Type Article in Periodical
Magazine / Source Plasmid
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WPF-4PYJF2P-1&_user=10&_coverDate=01%2F31%2F2008&_rdoc=2&_fmt=full&_orig=browse&_srch=doc-info(%23toc%236989%232008%23999409998%23677858%23FLA%23display%23Volume)&_cdi=6989&_sort=d&_docanchor=&_c
Field Microbiology, virology
Keywords Escherichia coli; Microcin C7; Plasmid copy number; Complete sequence
Description The complete sequence of the plasmid MccC7-H22 encoding microcin C7, isolated from probiotic E. coli H22, was determined and analyzed. DNA of pMccC7-H22 comprises 32,014 bp and contains 39 predicted ORFs. Two main gene clusters, i.e., genes involved in plasmid replication and maintenance and genes encoding microcin C7 synthesis, are separated by several ORFs homologous to ORFs present in IS (insertion sequence) elements and transposons. Additional 14 ORFs code for proteins with similarities to known proteins (4 ORFs) or for hypothetical proteins with unknown function (10 ORFs). The differences in G + C content of individual ORFs and gene clusters of pMccC7-H22 indicate a mosaic structure for the plasmid, resulting from recombination events.
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