Publication details

Determination of D- and L-amino acids produced by cyanobacteria using gas chromatography on Chirasil-Val after derivatization with pentafluoropropyl chloroformate

Authors

ZAHRADNÍČKOVÁ Helena HOLOUBEK Ivan

Year of publication 2007
Type Article in Periodical
Magazine / Source Analytical and Bioanalytical Chemistry
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords Amino acids; cyanobacteria; gas chromatography
Description A rapid and simple method was developed for the determination of free amino acids (AAs) released from cyanobacteria. The procedure involves trapping of AAs from the centrifuged cyanobacterial culture fluid on a cation-exchange resin, their release together with the resin by a direct treating with the reaction medium, followed by immediate derivatization with a respective chloroformate.
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