Publication details
Ektopická exprese organelových variant kukuřičné beta-glukozidázy Zm-p60.1 u transgenního tabáku
| Title in English | Ectopic expression in transgenic tobacco variants with different organel localization of maize beta-glucosidase Zm-p60.1 |
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| Authors | |
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | Bulletin České společnosti experimentální biologie rostlin a Fyziologické sekce Slovenské botanické společnosti |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | transgenic tobacco; cytokinin glucoside specific beta-glucosidase; Zm.p60.1 |
| Description | Cytokinins (CKs) form a class of plant growth regulators with key regulatory roles. The cytokinin metabolic network comprising biosynthesis, interconversion, conjugation and degradation is complex and has not been fully elucidated. Conjugation of CKs, the addition of low-molecular weight groups to the active molecule and thus reducing its activity is a common theme among plant growth regulators. cDNAs from maize and Brassica which encode beta-glucosidases that specifically cleave t-zeatin-O-glucoside (ZOG) have been identified. The native maize enzyme (Zm-p60.1) is located in coleoptile plastids and the over-expression in tobacco causes the plants to become hypersensitive to exogenous CKs. A recombinant version localised to the vacuole has been generated, and the over-expression of this variant abolishes the ability of transgenic tobacco to accumulate ZOG to levels matching the wild-type. Crosses between the two variants have been made and the simultaneous over-expression of the enzyme in both the chloroplast and the vacuole is expected to shed more light on the regulation of this branch of the CK metabolic pathway. Characterisation of the expression of the cDNA and protein, along with phenotype observations and responses of the crosses to exogenous CKs was presented. |
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