Publication details
v-Myb suppresses phorbol ester- and modifies retinoic acid-induced differentiation of human promonocytic U937 cells
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Periodical |
| Magazine / Source | Neoplasma |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | v-Myb; U937; differentiation; macrophage; phorbol ester; retinoic acid |
| Description | c-myb as well as v-myb oncogene code for transcription factors. They regulate transcription of specific target genes thus controling proliferation, differentiation and apoptosis of hematopoietic cells. Up-regulation of the c-myb expression or rearrangement/amplification of the myb locus are often involved in leukemogenesis. Enforced myb expression blocks differentiation of various leukemic cell lines. Human promonocytes U937 can be induced to differentiate to monocyte/macrophage-like cells using phorbol esters or to granulocytes using retinoic acid. In order to investigate transforming capability of v-myb, we expressed the v-myb oncogene of AMV in U937 cells. We found that v-Myb efficiently suppressed formation of macrophages upon treatment with phorbol ester. Some features of granulocytic differentiation of retinoic-acid-treated U937 cells were affected by the v-Myb protein as well. These results document that v-Myb is significantly involved in control of myeloid differentiation. |
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