Publication details
Průkaz identity kmenů izolovaných z povrchu zubů a ze zubních kanálků
| Title in English | Identity confirmation of bacterial strains isolated from dental caries and root canals |
|---|---|
| Authors | |
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | Mezinárodní seminář SVC |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | Streptococcus; Lactobacillus; identity confirmation; automatic ribotyping; whole-cell protein analysis |
| Description | A bacterial microflora of dental plaque and oral cavity is considered as an important factor in the formation and progression of dental carries. The bacteria that may penetrate through the damaged dental surface to the dental pulp and roots canal play an important role in a further progress of the dental carries due to their metabolic activity. The aim of this study was evaluation and comparison of three typing methods for typing of bacterial strains retrieved from the dental surface and root of identical teeth. Totally, 21 pairs of streptococci and lactobacilli (in total 42 strains) isolated from dental carries and root canals isolated from early childhood caries affected children were originally chosen based on the identical biotype and the results of rep-PCR fingerprinting with (GTG)5 primer. The selected isolates were additionally typed using whole-cell protein analysis and automatic ribotyping performed by RiboPrinter (DuPont Qualicon). The obtained results of the protein analysis and ribotyping congruently proved 11 strains pairs to be identical. Another two couples of the strains showed identical protein profiles while ribotyping results showed small differences and conversely, five pairs with the same ribotypes were not clearly consistent using the whole-cell protein analysis. Despite these different results and considering reproducibility values of both methods we can conclude that even these slightly different strains could be identical. Only three pairs of isolates retrieved from the same tooth were found as different based on clearly different ribotyping profile. Obtained results confirmed possibility of a penetration of the bacteria through the damaged dental surface to the root canals in a process of dental carries progression. A combination of used methods seems to be suitable for identity confirmation of the tested isolates. The study was supported by project n. 1M0528 of the Ministry of Education of the Czech Republic. |
| Related projects: |