Publication details

A label free piezoelectric immunosensor for rapid assay of Escherichia coli

Authors

POHANKA Miroslav SKLÁDAL Petr PAVLIŠ Ota

Year of publication 2008
Type Article in Periodical
Magazine / Source Journal of Immunoassay and Immunochemistry
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Piezoelectric biosensor; Quartz crystal microbalance; Immunoassay; Immunosensor; Escherichia coli
Description piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of the piezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 106-109 CFU/mL; signal of the negative control was not statistically relevant in the selected range. Samples could be analyzed in four minutes and one measuring cycle including regeneration was completed within ten minutes. Repeatability of the developed method is discussed; the signal obtained from three different biosensors was 12.9+/-0.4 Hz for the sample containing 108 CFU/mL.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info