Publication details

Metallothionein levels in sperm of various fish species

Authors

FABRIK Ivo SVOBODOVÁ Zdenka ADAM Vojtěch KŘÍŽKOVÁ Soňa TRNKOVÁ Libuše BEKLOVÁ Miroslava RODINA M. KIZEK René

Year of publication 2008
Type Article in Periodical
Magazine / Source JOURNAL OF APPLIED ICHTHYOLOGY
MU Faculty or unit

Faculty of Science

Citation
Field Electrochemistry
Keywords fish sperm; heavy metals; chronopotentiometrzy
Description In the present paper we determined low molecular heavy metal binding protein called metallothionein (MT) in fish spermatozoa sampled from four fish species (Acipenser baerii, Acipenser ruthenus, Perca fluviatilis and Sander lucioperca). The spermatozoa samples were analyzed with differential pulse voltammetry DPV (Brdicka reaction). The average content of MT in the fish sperm was 0.64 +- 0.55 micro m. The highest content of MT was determined in spermatozoa of S. lucioperca (0.96 +- 0.86 micro m), followed by P. fluviatilis (0.88 +- 0.11 micro m), A. ruthenus (0.57 +- 0.60 micro m) and A. baerii (0.43 +- 0.25 micro m). The differences in the content of MT was probably related to the differences in age and perhaps to the health status based on the environmental conditions in which the tested specimens developed while also other still unknown factors may have been involved.
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