Publication details
Application of a new method for electroosmotic flow measurement to coating characterization in capillary electorphoresis of proteins
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | 11th International Symposium on Separation Sciences ISSS 2005. Book of Abstracts |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | electroosmotic flow measuremet; electrophoresis of proteins; capillary coating |
| Description | The quality of the inner surface of capillaries in CE is critical. Problems come mainly from adsorption of the peptides (proteins) on the surface and due to the fact the biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte. Therefore, modification (coating) of the fused silica capillary is often necessary for separation of proteins and peptides. |